Department of PhysicsDepartment of Biomedical Engineering, Purdue University
Coffee and doughnuts served at 3:15pm.
Coherent Raman microscopy is a powerful bioimaging tool owing to its inherent 3D submicron resolution, extended penetration depth, real-time data acquisition, and compatibility with other non-linear optical modalities. First part of the presentation will focus on application of coherent anti-Stokes Raman scattering microscopy, including study of triglyceride metabolism in enterocytes of mice, non-invasive rapid assay of complex genotype-phenotype relationships in a single living C. elegans, and in vivo study of lipid metabolism in drosophila larvae. In the second part of the talk the potential applications of novel coherent technique, Stimulated Raman scattering, will be discussed and exemplified on lipid metabolism in cells, 3-D cell culture imaging, and lipid trafficking in drosophila embryo.